Journal: PLOS One

Article Title: DJ-1 alleviates high glucose-induced podocyte injury via activating ERK1/2 signaling

doi: 10.1371/journal.pone.0346714

Figure Lengend Snippet: (A) Representative Western blot images and quantitative statistical bar graphs of protein expression levels of DJ-1 in HPCs treated with mannitol (MG) or D-glucose were analyzed by Western blotting analysis and Image J software, respectively. (B) The mRNA levels of DJ-1 in HPCs treated with MG or D-glucose were analyzed by qRT-PCR analysis. (C) The cell viability was measured by CCK-8 assays in HPCs treated with MG, LG, HG and HG + DJ-1, respectively. (D) The cell viability was analyzed by EdU assays in HPCs treated with MG, LG, HG and HG + DJ-1, respectively. (E) The cell apoptosis was analyzed by Annexin V-FITC/PI apoptosis assay in HPCs treated with MG, LG, HG and HG + DJ-1, respectively. (F) The apoptotic rate of HPCs treated with MG, LG, HG and HG + DJ-1 was analyzed by GraphPad Prism 8 software. (G) Representative Western blot images and quantitative statistical bar graphs of protein expression levels of cleaved caspase 3 and Bcl2 in HPCs treated with LG, HG and HG + DJ-1 were analyzed by Western blotting analysis and Image J software, respectively. MG: mannitol, LG: 5.5mM D-glucose, MG: 15mM D-glucose, HG: 30mM D-glucose, DJ-1: transfected with 1 μg/mL DJ-1-Flag-pcDNA3.1 plasmid. Data are shown as mean ± SD and representative of three independent experiments. * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: Annexin V-fluorescein isothiocyanate/propidium iodide (Annexin V-FITC/PI) apoptosis detection kit (HY-K1073, MCE, NJ, USA) was carried out to measure HPC apoptosis.

Techniques: Western Blot, Expressing, Software, Quantitative RT-PCR, CCK-8 Assay, Apoptosis Assay, Transfection, Plasmid Preparation

Journal: PLOS One

Article Title: DJ-1 alleviates high glucose-induced podocyte injury via activating ERK1/2 signaling

doi: 10.1371/journal.pone.0346714

Figure Lengend Snippet: (A) Representative Western blot images and quantitative statistical bar graphs of protein expression levels of NF-κB p65 and AP-1 in HPCs treated with LG, HG, HG + DJ-1 and HG + DJ-1 + temuterkib were analyzed by Western blotting analysis and Image J software, respectively. (B) Representative Western blot images and quantitative statistical bar graphs of protein expression levels of cleaved caspase 3 and Bcl2 in HPCs treated with LG, HG, HG + DJ-1 and HG + DJ-1 + temuterkib were analyzed by Western blotting analysis and Image J software, respectively. (C) The cell viability was measured by CCK-8 assays in HPCs treated with LG, HG, HG + DJ-1 and HG + DJ-1 + temuterkib, respectively. (D) The cell viability was analyzed by EdU assays in HPCs treated with LG, HG, HG + DJ-1 and HG + DJ-1 + temuterkib, respectively. (E) The cell apoptosis was analyzed by Annexin V-FITC/PI apoptosis assay in HPCs treated with LG, HG, HG + DJ-1 and HG + DJ-1 + temuterkib, respectively. (F) The apoptotic rate of HPCs treated with LG, HG, HG + DJ-1 and HG + DJ-1 + temuterkib were analyzed by GraphPad Prism 8 software. MG: mannitol, LG: 5.5mM D-glucose, MG: 15mM D-glucose, HG: 30mM D-glucose,temuterkib: 1μM, DJ-1: transfected with 1 μg/mL DJ-1-Flag-pcDNA3.1 plasmid. Data are shown as mean ± SD and representative of three independent experiments. * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: Annexin V-fluorescein isothiocyanate/propidium iodide (Annexin V-FITC/PI) apoptosis detection kit (HY-K1073, MCE, NJ, USA) was carried out to measure HPC apoptosis.

Techniques: Western Blot, Expressing, Software, CCK-8 Assay, Apoptosis Assay, Transfection, Plasmid Preparation

Journal: Frontiers in Pharmacology

Article Title: The tryptanthrin derivative g2 suppresses tongue squamous cell carcinoma via PI3K/AKT/P53 cathway modulation: evidence from in vitro and in vivo studies

doi: 10.3389/fphar.2026.1789002

Figure Lengend Snippet: Effect of g2 on apoptosis in CAL-27 and SCC9 cells. Cells were treated with increasing concentrations of g2 (1, 2, and 5 μM) for 24 h. Apoptosis was assessed by Annexin V/PI double staining using flow cytometry. (A) Representative flow cytometry plots of CAL-27 and SCC9 cells. The lower-right quadrant (Q1-LR) indicates early apoptotic cells (Annexin V + /PI − ), and the upper-right quadrant (Q2-UR) indicates late apoptotic/necrotic cells (Annexin V + /PI + ). Numbers represent the percentage of cells in each quadrant. (B) Quantification of total apoptotic cells (early + late) from three independent biological replicates (n = 3 per group). Data are shown as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 vs. control group.

Article Snippet: The quantification of apoptotic CAL-27 and SCC9 cells was conducted utilizing the Annexin V-fluorescein isothiocyanate (FITC) apoptosis detection kit (Invitrogen, Carlsbad, CA, United States).

Techniques: Double Staining, Flow Cytometry, Control

Journal: Cancer Medicine

Article Title: Improved Chemosensitivity in Metastatic Castration‐Resistant Prostate Cancer: The Synergistic Effects of S‐Adenosylmethionine and Cabazitaxel

doi: 10.1002/cam4.71784

Figure Lengend Snippet: Effect of AdoMet, CBZ and their combination on apoptosis in mCRPC cells. (A) DU 145 was treated with 400 μM AdoMet and 0.7 nM CBZ and (C) PC‐3 was treated with 400 μM AdoMet and 1.5 nM CBZ, alone or in combination for 72 h. Apoptosis was evaluated by FACS analysis. Representative dot plots of both Annexin V‐FITC and PI‐stained cells. The different quadrants show the percentage of cells: Viable cells, lower left (Q4); early apoptotic cells, bottom right (Q3); late apoptotic cells, top right (Q2); non‐viable necrotic cells, upper left (Q1). For each sample, 2 × 10 4 events were acquired. Analysis was carried out by triplicate determination of at least 3 separate experiments. Data represents the average of three independent experiments. The protein levels of PARP‐1 in (B) DU 145 and (D) PC‐3 were detected by Western blot analysis using total cell lysates. The house‐keeping protein α ‐tubulin was used as loading control. All data represents the average of three independent experiments. The images are representative of three immunoblotting analyses obtained from three independent experiments ± SD (** p < 0.01).

Article Snippet: The Annexin V‐fluorescein isothiocyanate (V‐FITC) apoptosis detection kit (Catalog No. 556547) was provided by eBioscience (San Diego, CA, USA).

Techniques: Staining, Western Blot, Control